By Sidney Fleischer, Becca Fleischer (Eds.)
The seriously acclaimed laboratory normal, Methods in Enzymology, is likely one of the so much hugely revered courses within the box of biochemistry. considering the fact that 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct at the present time - actually a vital book for researchers in all fields of existence sciences
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Additional info for Biomembranes Part M
20 G. F. Azzone, T. Pozzan, S. Massari, and L. Pregnolato, Biochim. Biophys. Acta 501, 307 (1978). zJ H. Woelders, W. J. Van der Zande, A. A. F. Colen, R. J. A. Wanders, and K. Van Dam, FEBS Lett. 179, 278 (1984). 22 p. Mitchell and J. Moyle, Eur. J. Biochem. 4, 530 (1968). 23 A. Alexander, B. Reynatarje, and A. L. Lehninger, Proc. Natl. Acad. Sci. A. 75, 5296 (1978). 24 F. Sholtz, I. A. Gorskaya, and A. V. Kotelnikova, Eur. J. Biochem. 136, 129 (1983). 25 M. C. Sorgato, S. J. Ferguson, D. B. Kell, and P.
In order to maintain optimal mitochondrial respiratory control, it is critical that the heart tissue from each animal be isolated and minced within 30 sec after decapitation and that all subsequent operations be performed at 0-4 °. The combined mince is washed with three, 30 ml volumes of MSB medium, resuspended in approximately 10 ml of MSB per gram of heart, and 200 IU of collagenase is added. 117 mm (caliper measurement). The homogenate is allowed to incubate on ice for 1 min, and EGTA is added to a final concentration of 1 mM to halt collagenolysis and prevent mitochondrial uptake of Ca(II).
G. Van den Bergh, this series, Vol. 10, p. 749. 30 G. Woldegiorgis, S. Y. K. Yousufzai, and E. Shrago, J. Biol. Chem. 257, 14783 (1982). 31 M. A. K. Markwell, S. M. Haas, N. E. Tolbert, and L. L. Bieber, this series, Vol. 72, p. 296. L.... /Ix / ~ / X SA? min at 8 ° ° ° g / / / SB2 PB2 Sj (Discard) I CI A Resuspend L B Centrifuge for IOmin Y Poo P, 4- P at 8000g j . # . JA Resuspend Po~ P,-- 7 f i - NB Centrifuge for IOmin c, ~ ? ~ . SA3 (Discard) 8OOOg ~ PA3 0 r PA3 , Final Mitochondria Pellet FIo.
Biomembranes Part M by Sidney Fleischer, Becca Fleischer (Eds.)