Download e-book for kindle: Biochemical and Structural Dynamics of the Cell Nucleus by Eugenia Wang

By Eugenia Wang

ISBN-10: 0127345752

ISBN-13: 9780127345758

ISBN-10: 0323157882

ISBN-13: 9780323157889

Biochemical and Structural Dynamics of the phone Nucleus

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C. Muscle-Specific Inhibition of DMSO Treatment We were able to distinguish between the possibilities that DMSO is inhibiting all protein expression nonspecifically, or whether the inhibition is musclespecific by 2D gel autoradiography of [ ^**S]methionine labeled control and DMSO treated myogenic cultures. As we can see in Figure 6, based on the decrease in the synthesis of the muscle-specific isoforms of myosin light chains, tropomyosins, and α-actin, and the lack of inhibition observed in the expression of nonmuscle proteins such as vimentin, it appears that DMSO inhibition is primarily muscle-specific.

To further investigate the suggestion of a role for lamin A in the regulation of muscle-specific gene expression, we have examined the expression of lamin A during the inhibition of myogenic differentiation. We have found that the presence of the Ca"*"*" chelator EGTA, interfered with the accumulation of muscle-specific proteins, but not lamin A. Treatment of myogenic cells with 2% dimethyl sulfoxide (DMSO) resulted in the reversible inhibition of lamin A and muscle-specific protein accumulation.

Cell 49:515-526. , C. Comeau and M. Benson 1984. Organization and modulation of nuclear lamina structure. J. Cell Sei. Suppl. 1:137-160. , C. Chiu, A. Minty, and H. Blau. 1986. The pattern of actin expression in human fibroblasts χ mouse muscle heterokaryons suggests that human muscle regulatory factors are produced. Cell 47:123-130. , and W. Earnshaw. 1986. Topoisomerase II: a specific marker for cell proliferation. J. Cell Biol. 103:2569-2581. , E. Debus, M. Osborn, K. Weber, and W. Franke. 1984.

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Biochemical and Structural Dynamics of the Cell Nucleus by Eugenia Wang

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