Get Artificial Intelligence in Label-free Microscopy. Biological PDF

By Ata Mahjoubfar, Claire Lifan Chen, Bahram Jalali

ISBN-10: 3319514482

ISBN-13: 9783319514482

This e-book introduces time-stretch quantitative section imaging (TS-QPI), a high-throughput label-free imaging stream cytometer constructed for giant facts acquisition and research in phenotypic screening. TS-QPI is ready to seize quantitative optical section and depth pictures at the same time, permitting high-content phone research, melanoma diagnostics, custom-made genomics, and drug improvement. The authors additionally exhibit an entire computing device studying pipeline that plays optical part dimension, photograph processing, characteristic extraction, and category, allowing high-throughput quantitative imaging that achieves checklist excessive accuracy in label -free mobile phenotypic screening and opens up a brand new route to data-driven diagnosis.

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The complementary metal–oxide–semiconductor (CMOS) camera [44], and hence its scan rate is limited by the frame rate of the camera (up to 10 kHz) [44] and also the trade-off between the number of pixels and frame rate. In this chapter, we propose and demonstrate a laser vibrometer that overcomes the limitations in the conventional multi-dimensional laser vibrometers and achieves high-speed imaging-based surface vibration measurements with nanometer-scale axial resolution at 100 times higher scan rates than the conventional methods.

4 kHz. As an illustrative demonstration, we captured vibrations of a reflective diaphragm oscillating at 1 kHz (Fig. 6). Finally, as an example of the HDLS’ biomedical utility, we demonstrated highprecision high-throughput flow cytometry using the HDLS. Low spatial resolution of conventional flow cytometers causes a considerable number of false positive events that result in statistical error in subpopulation analysis. ) (Fig. 7a). 3 Applications of Hybrid Dispersion Laser Scanner 25 Fig. 3 Wavelength-to-time mapping with dispersive Fourier transformation.

7 MHz scan rate. While we performed 1D cross-sectional imaging in this proof-of-principle demonstration, the technique can naturally be extended to 2D by using a 2D spatial disperser [10, 48]. Chapter 4 Three-Dimensional Ultrafast Laser Scanner Laser scanners are essential for scientific research, manufacturing, defense, and medical practice. , galvanometric mirrors and acousto-optic deflectors) falls short for many applications, resulting in motion blur and failure to capture fast transient information.

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Artificial Intelligence in Label-free Microscopy. Biological Cell Classification by Time Stretch by Ata Mahjoubfar, Claire Lifan Chen, Bahram Jalali

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